Mass Spectrometry

The proteomics core facility offers mass spectrometry services to the NTU community and beyond. We provide a wide range of services including determination of molecular weight for biomolecules and protein identification from various sources. We hope to expand further in the near future.

To request for a service,
  • Fill up and email the sample submission form to [email protected]
  • Print and attach a hard copy of the form to each sample and submit it to Meng Wei at the mass spectrometry room.
We are committed to provide high quality service at an affordable cost, with fast turnaround under normal instrumental conditions. Protein (unlabeled) identification from coomassie blue-stained gel bands is guaranteed. You will not be charged the service if the results turn out otherwise.

Please see the list of equipment available within the facility below.
List of equipment in Mass Spec Facility

​CONTACT INFORMATION

Location of FacilitySchool of Biological Sciences
SBS-03N-S1 (Mass Spectrometry Room)
Contact Details​​(Ms) Meng Wei
Email Address: [email protected]
Contact No.:(65) 6592 2639
Associate Professor Jaume Torres
Email Address: [email protected]
Contact No.: (65) ​6316 2857

SAMPLE SUBMISSION FORMS

CHARGES FOR MASS SPECTROMETRY SERVICE

Service providedNTU UsersOther academic usersNon-academic users
Protein ID by MALDI-TOF/TOF$100/sample$160/sample$240/sample
Protein ID by LC-MS/MS$210/sample$300/sample$440/sample
4-plex iTRAQ with reagent and sample preparation$3,000$4,500$6,000
6-plex TMT with reagent and sample preparation$4,100$6,150$8,200
10-plex TMT with reagent and sample preparation$4,800$7,200$9,600
Whole proteome ID (including sample preparation, HPLC fractionation)$2,500$3,750$5,000
Phosphopeptide enrichment and analysis per protein $300
(sample preparation $150) 		$400 $600
per phosphor-proteome$2500
(sample preparation $500) 		$3750$5000
N-Glycopeptide enrichment and analysis$300/protein; $2500/glycol-proteome$400/protein; $3750/phosphor-proteome$600/protein; $5000/phosphor-proteome
SILAC (including HPLC fractionation)per sample$350$500$700
per proteome$2500$3500$5000
Service providedNTU UsersOther academic usersNon-academic users
Pro​tein ID by MALDI-TOF/TOF$30 / sample$60 / sample$100 / sample
Protein ID by LC-MS/MS$150 / sample$200 / sample$300 / sample
Molecular Weight determination by MALDI-TOF/TOF$20 / sample$30 / sample$40 / sample
Molecular Weight determination by LTQ-FT$75 / sample$100 / sample$150 / sample
Service providedNTU UsersOther academic usersNon-academic users
Desalting / Sample concentration$20 / sample$25 / sample$40 / sample
Service providedNTU UsersOther academic usersNon-academic users
MALDI-TOF/TOF performed by user $160 / hour--
Service providedNTU UsersOther a​​cademic usersNon-academic users
SDS PAGE/sample purification$150 / gel$200 / gel$300 / gel

Note

  • Service charge may be changed at the discretion of the School of Biological Sciences without prior notice.
  • All service charges are before GST.
  • For proteome / iTRAQ / TMT samples, the price is for running once, additional charges apply if additional repeat service is required. Repeat services will be charged at a 50% discounted rate.
  • If the LCMS sample converted from MALDI-TOF has a sample quantity of more than 10, service provided will be charged at a 20% discounted rate. If the sample quantity is more than 20, service provided will be charged at a 30% discounted rate.
  • If the Normal LCMS sample has a sample quantity of more than 10, service provided will be charged at a 10% discounted rate. If the sample quantity is more than 20, service provided will be charged at a 20% discounted rate


FAQ

Please refer to the table of charges
Package service of protein ID by MALDI-TOF/TOF (under packaged service) will be a suitable service for you.
It includes Reduction/Alkylation, In-gel or in solution digestion by trypsin, desalting / sample concentration by Ziptip, Running sample by instrument and database searching.
For protein ID service, customers could submit samples in gel band format or solution format. For MW determination, customers could submit samples in solution format.
No. We do not provide CAF sequencing service.
We usually use NCBInr or Swiss Prot database