CFX96 Touch - Real-Time PCR Detection System

Differential Scanning Fluorimetry (DSF) is a high throughput screening method for protein solutions using interactions of proteins with low-molecular-weight ligands. The technique relies on using specific dyes that fluoresce as they interact with proteins in their denaturation transition between a strongly hydrated solvated state and the final aggregated solid state. Sypro orange is well suited as a dye of choice as it was designed specifically to activate fluorescence when binding to hydrophobic patches exposed as a result of conformational changes. Using such a specific dye, DSF elicits and identifies key temperature transitions and protein stability features, which in turn allows the probing and analysis of molecular interactions. During a DSF experiment, denaturation is controlled by slowly heating the protein solution from a base-line, natural temperature. This heating induces changes in the conformation of the protein. Specifically designed dyes can interact with the bonds and signal this interaction by changes in their fluorescence spectrum. Such information on transitions is of fundamental importance for better understanding a protein’s biochemistry, its structure-function relationships and its resistance to chemical, mechanical, and thermal denaturation.

CFX96 

• Temperature range: 0°C – 100°C
• Gradient: 30°C - 100°C (operational range) and 1°C - 24°C (programmable span) 
• Range of excitation/emission wavelengths (nm): 450nm - 730nm