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Biomolecular Interactions
The Biomolecular Interactions (BMI) Platform is the solution to characterize molecules of your research and interest. Our techniques are suitable for protein to protein or DNA / ligand / small molecules / hormones / fragments interactions and more.
PR Instruments The SPR technology in Biacore systems is used to monitor binding events between molecules ranging from ions to viruses. The technology provides binding kinetics, affinity, specificity and concentration, without any needs for labels. We have the state of the art Biacore T200 and the standard workhorse Biacore 3000 available. With this technology, we can help you characterize interactions between - Protein to Protein - Protein to DNA - Protein to Sugar - Protein to small molecular / drug / fragments / hormones - Antibody interactions - Affimers - And many more! As long as the binding partners are soluble and there are means to couple one of them onto a sensor chip surface. Through this technique, you can determine the strength of interactions, as well as the real time kinetics, i.e. the association and dissociation rates, which can allow you to reach new research conclusions.
For more information, visit Biomolecular Interactions page
At this facility, we offer the following technologies:
- Surface Plasmon Resonance Interaction Assays on a Biacore T200 / Biacore 3000 - Isothermal Calorimetry on a Malvern ITC200
- Dynamic Light Scattering on a Malvern Zetasizer ZS Nano
- Circular Dichroism measurements on a Chirascan CD Spectrometer
- Fluorescence measurements on a Cary Eclipse Fluoroscent Spectrophotometer.
- Analytical Ultra-Centrifugation on a Beckman Proteomelab-XL
| Equipment | Cost Scheme | |
|---|---|---|
| NTU | Other institutions/Industry | |
| Biacore T200 (assisted) | $150/day + chip(s) | $250/day + chip(s) |
| Biacore 3000 (assisted) | $130/day + chip(s) | $230/day + chip(s) |
| ITC200 | $130/day | $250/day |
| Malvern DLS | $10/hour | $40/hour |
| Chriascan CD | $20/hour | $80/hour |
| Cary Eclipse Fluorospect | $20/hour | $40/hour |
| Analytical UC | $70/hour (trained user) $120/day (assisted) | $250/day (assisted) |
- SPR Instruments
- SPR Technology
- Circular Dichroism
- Dynamic Light Scattering
- Isothermal Titration Calorimetry