Research Capabilities
State of the art facilities encompassing of the following areas:
Microscopy Facility
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- Confocal laser scanning fluorescence microscopy
- Light Sheet fluorescence microscopy
- Live cell fluorescence imaging
- Total Internal Reflection Fluorescence (TIRF) imaging
- Epifluorescence stereomicroscope imaging
Mass Spectrometer
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- MALDI-TOF/TOF
- LC-MS/MS
- 4-plex iTRAQ
- 6-plex and 10-plex tandem mass tags (TMT)
- Whole proteome identification
- Phosphopeptide enrichment and analysis
- N-glycopeptide enrichment and analysis
- SILAC metabolic labelling system
Flow Cytometry
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- High throughput analysis of up to 5 colours
- High throughput cell sorting
Biomolecular Interaction Platform
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- Biacore Surface Plasmon Resonance system
- Isothermal titration microcalorimeter
- Dynamic Light Scattering system
- Circular dichroic spectrometer
- Analytical ultracentrifuge
- Fluorescence spectrophotometer with fluorescence, phosphorescence, chemiluminescence, and bioluminescence capabilities
Protein Production Platform
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- High-throughput cloning and testing of protein expression constructs, with Escherichia coli as the main workhorse
- Mammalian and insect cell systems for more difficult target proteins
- Advice and suggestions for designing expression constructs
- Liter-scale cultures and medium-throughput protein purification using FPLC systems
- Project management and data sharing on a laboratory information management system
Nuclear Magnetic Resonance
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- Solution state NMR ranging from 400 to 700 MHz
- Biomolecular structural, interaction, and functional studies