Protein Production Platform​

Do you want to get your hands on a recombinant version of your favorite protein? 
Established in 2009, the Protein Production Platform (PPP) has to date produced more than 4,000 recombinant protein batches for both academic and industrial partners. Our strategy is simple, robust and allows us to work in parallel with many different proteins and protein constructs.
 

Your target will be sub-cloned by Ligation Independent Cloning and expression screened in either E.coli, insect or mammalian cells depending on your preference and needs. We heavily utilize the multiple-construct approach in order to identify well-behaved protein constructs that can be scaled up with ease. Constructs that are deemed to express well will be scaled up and purified. As all constructs carry a hexa-histidine tag they are typically purified through a two-step process (immobilized metal-ion affinity chromatography followed by size exclusion chromatography).

Protein Production Platform​

  • As a collaborator of the PPP, you will have the option to provide us with your own cDNA template or choose your selected target from our human cDNA collection (Lamesh et al, 2006).
  • As a collaborator of the PPP, you can follow the progress of your target by logging into our LIM system where the results will be displayed in an interactive and user friendly way.
  • As part of our transparent workflow, we will communicate your results to you clearly and directly.
If ​you are interested in our protein production services, please visit https://proteins.sbs.ntu.edu.sg/login.php and read more about our services and strategy.

CONTACT INFORMATION

Location of FacilitySchool of Biological Sciences
SBS-04S-43
Contact Details
(For more information, price inquiries and project discussions) 		(Dr) Chen Ming Wei
Email Address: [email protected]
Contact No.: 65137461