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Membrane Compartmentalisation and Cell Polarity Lab
DESCRIPTION OF LAB RESEARCH WORK
We are interested in the molecular, structural, and functional organization of the cell membrane. Our overarching goal is to understand how compartmentalization of the cell cortex regulates cellular form and function and complex cell behaviors such as cell migration and polarity, in particular in epithelial cells. We combine cell biological, biochemical, structural and functional approaches with high resolution imaging including light and electron microscopy and correlative imaging.
 | LEAD PI
Alexander Ludwig Assistant Professor Email: [email protected] Phone: (65) 6908 2212 Office: EMB-06-09 Lab webpage |
 | Linda Woo Jiabao Project Officer Email: [email protected] Phone: (65) 6908 2213 |
 | Eleanor Martin
Research Fellow Email: [email protected] Phone: (65) 6908 2213 |
 | Barbara Huebner
Research Associate Email: [email protected] Phone: (65) 6908 2213 |
 | Siti Maryam Jameelah Binte Mohd Yatim
Research Associate Email: [email protected] Phone:(65) 6908 2213 |
 | Hana Maldivita Tambrin
PhD student Email: [email protected] Phone: (65) 6908 2213 |
 | Sujasha Ghosh
PhD student Email: [email protected] Phone: (65) 6908 2213 |
- Nanometer-scale organisation of the epithelial cell cortex
- Functional characterisation of novel polarity regulators in mammalian epithelial cells
- Correlative light and electron microscopy of membrane architecture
Full list of publications can be found here
- Tan, B, Peng, S, Yatim, SMJM, Gunaratne, J, Hunziker, W, and
Ludwig, A#. An optimised protocol for proximity biotinylation in
confluent epithelial cell cultures using the peroxidase APEX2. STAR
Protocols. Published July 31 2020 doi:
https://doi.org/10.1016/j.xpro.2020.100074. #corresponding author
- Tan, B, Yatim, SMJM, Peng, S, Gunaratne, J, Hunziker, W, and Ludwig, A#. The mammalian Crumbs complex defines a distinct polarity domain apical of epithelial tight junctions. Current Biology. Published June 11 2020. doi: https://doi.org/10.1016/j.cub.2020.05.032. #corresponding author
- Ludwig, A#. Selective Visualization of caveolae by TEM using APEX2. Caveolae - Methods and Protocols. Methods in Molecular Biology. 2020;2169:1-10. doi: https://doi.org/10.1007/978-1-0716-0732-9. #corresponding author
- Tie, HC, Ludwig, A, Sandin, S, Lu, L. The Spatial Separation of Processing and Transport Functions to the Interior and Periphery of the Golgi Stack. Elife. 2018 Nov 30;7. pii: e41301. doi: https://doi.org/10.7554/eLife.41301.
- Ludwig, A#, Nguyen, TH, Leong, D, Ravi, LI, Huan, TB, Sandin, S & Sugrue, R#. 2017. Caveolae provide a specialized membrane environment for respiratory syncytial virus assembly. J Cell Sci. 2017 Feb 2. doi: https://doi.org/10.1242/jcs.198853. #corresponding author.
- Ludwig, A#, Nichols, BJ, Sandin, S. Architecture of the Caveolar Coat Complex, J Cell Sci. 2016 Jul, doi: https://doi.org/10.1242/jcs.191262. #corresponding author.
- Shvetz E, Ludwig A, Nichols BJ. News from the caves: update on the structure and function of caveolae. Curr. Opin. Cell Biol. 2014 Jun 5; 29C:99-106. doi: https://doi.org/10.1016/j.ceb.2014.04.011.
- Ludwig A#, Howard G, Mendoza-Topaz C, Deerinck T, Mackey M, Sandin S, Ellisman, MH, and Nichols B#. Molecular Composition and Ultrastructure of the Caveolar Coat Complex. PLoS Biology. 2013 Aug;11 (8), e1001640. Completed doi: https://doi.org/10.1371/journal.pbio.1001640. #corresponding author.
- Ludwig A*, Otto GP*, Riento K, Hams E, Fallon PG, Nichols BJ. Flotillin microdomains interact with the cortical cytoskeleton to control uropod formation and neutrophil recruitment. J Cell Biol. 2010 Nov;15;191(4):771-81. doi: https://doi.org/10.1083/jcb.201005140. *equal contribution.