Microscopy

DESCRIPTION The Zeiss LSM710 system is an inverted point-scanning microscope that is ideal for research in cell and molecular biology. It provides great sensitivity and low background noise during image acquisition. With the Airyscan detector, one can achieve 1.7x higher resolution in all three spatial dimensions. It is suitable for 3D optical sectioning/z-sectioning, co-localization studies, live cell imaging, photoactivation or photobleaching experiments such as Fluorescence Recovery After Photobleaching (FRAP) and Förster Resonance Energy Transfer (FRET).

SPECIFICATIONS
Microscope Body

• Axio Observer.Z1
• Environmental Chamber for temperature control

Objectives

• EC Plan-NeoFluar 10x/0.30
• EC Plan-NeoFluar 20x/0.50
• EC Plan-NeoFluar 40x/1.30 Oil DIC
• Plan-Apochromat 63x/1.40 Oil DIC
• EC Plan-NeoFluar 100x/1.3 Oil

Light Source

• Halogen lamp for transmitted light
• X-Cite 120 illuminator for reflected light

Lasers

• Argon (458,488,514nm)
• HeNe 633nm
• DPSS 561nm
• Diode 405nm

Filter Sets

Filter set	Ex	Em
Zeiss Filter set 38 (GFP)	BP 470/40	BP 525/50
Zeiss Filter set 43 (dsRed)	BP 550/25​​	BP 605/70
Zeiss Filter set 49 (DAPI)	G365nm	BP 445/50

Detectors

• 2 standard PMTs
• Airyscan detector

Software

• Zen 2.1 (black)

DESCRIPTION The Zeiss LSM510 meta system is an inverted point-scanning microscope that is ideal for most standard fluorescent microscopy applications. This system features a variable pinhole that can be controlled to eliminate out-of-focus light and has the ability to collect serial optical sections from thick specimens.

SPECIFICATIONS
>Microscope Body

• Axiovert 200M

Objectives

• Plan-NeoFluar 10x/0.30
• Plan-Apochromat 20x/0.6 Ph2
• LD Achroplan 40x/0.6 corr Ph2
• Plan-Apochromat 63x/1.40 Oil DIC
• Plan-NeoFluar 100x/1.3 Oil Ph3

Light Source

• Halogen lamp for transmitted light
• HBO100 for reflected light

Lasers

• Argon (458,488,514nm)
• HeNe 543nm
• HeNe 633nm
• Diode 405nm

Filter Sets

Filter set	Ex	Em
Zeiss Filter set 01 (Blue)	BP 365/12	LP 397
Zeiss Filter set 09 (Green)	BP 450-490	LP 515
Zeiss Filter set 15 (​Red)	BP 546/12	LP 590

Detectors

• 3 photodetectors, namely the meta detector (polychromatic detector) and 2 conventional photomultiplier tubes.

Software

• Zen 2009

DESCRIPTION
The Zeiss Laser TIRF3 system is well suited for visualization of structures or molecular processes at or near cell membranes via the Total Internal Reflection Fluorescence (TIRF) principle. When properly configured, this imaging technique can result in narrow imaging depths of less than 100 nm from a glass-specimen interface. Single molecule dynamic processes in cell-free systems may also be observed.

Microscope Body

• Axio Observer.Z1

Objectives

• EC Plan-NeoFluar 10x/0.30
• a-Plan Apochromat 100x/1.46 Oil DIC

Light Source

• Halogen lamp for transmitted light
• HXP120 for reflected light
• Solid state laser 488nm and 561nm

Filter Sets

Filter set	Ex	Em
Zeiss Filter set 52	BP 488/10	BP 525/50
Zeiss Filter set 74	DBP 480/30	DBP 525/31
Zeiss Filter set 86	BP 556/25	BP 630/98

Detectors

• EMCCD Evolve 512

Software

• Zen blue

DESCRIPTION
The Zeiss Lightsheet imaging system enables you to obtain optical sections of large samples, with virtually no phototoxicity or bleaching and with high temporal resolution and very fast rates.

This system is suitable for performing:

• Fluorescence imaging of spatio-temporal patterns within cells during embryogenesis of model organisms such as Zebrafish and Drosophila melanogaster.
• Fast imaging of cellular dynamics in embryos and small organisms like cell migration, cardiac development, blood flow, calcium imaging etc.
• Live imaging of 3D cell cultures, spheroids, cysts, tissue and organotypic cultures.
• Imaging of optically cleared specimens

Microscope Body

• Axio Observer.Z1

Objectives

• Lightsheet Z.1 10x/0.2 (illumination)
• W Plan-Apochromat 40x/1.0
• W Plan-Apochromat 63x/1.0

Light Source

• Solid state laser 405nm, 488nm and 561nm

Detectors

• CCD camera

Software

• Zen 2014 SP1 (black edition)

DESCRIPTION
The Zeiss Live Cell Observer system is an inverted widefield fluorescence microscope. It is equipped with a temperature-controlled incubation system suitable for live cell time-lapse imaging experiments.

Microscope Body

• Axiovert 200M

Objectives

Light Source

• halogen lamp for transmitted light
• X-cite 120 illuminator for reflected light

Filter Sets

Filter set​​	Ex	Em
Zeiss Filter set DAPI		BP 445/50
Zeiss Filter set GFP	BP 470/40	BP 525/50
Zeiss Filter set TRITC	BP 550/25	BP 605/70
Zeiss filter set Cy5	BP 640/30	BP 690/50

Detectors

• CCD camera

Software

• Axiovision 4.8
• software

• Plan-Neofluar 10x/0.3
• Plan-Neofluar 20x/0.5
• LD Plan-NeoFluar 40x/0.6
• Plan-Apochromat 63x/1.4 Oil DIC4

DESCRIPTION
The Zeiss Live Cell Observer system is a state-of-the art inverted widefield fluorescence microscope. It is equipped with a temperature-controlled CO2 incubation system for live cell time-lapse imaging experiments. Suitable for tile and multi-position imaging.

Microscope Body

• Axio Observer.Z1
• Environmental Chamber for temperature control

Objectives

• EC Plan-NeoFluar 10x/0.30
• EC Plan-NeoFluar 20x/0.50
• EC Plan-NeoFluar 40x/0.75
• Plan-Apochromat 63x/1.40 Oil DIC
• Plan-Apochromat 100x/1.4 Oil

Light Source

• Halogen lamp for transmitted light
• X-Cite 120 illuminator for reflected light

Filter Sets

Filter set	Ex	Em
Zeiss Filter set 38 (GFP)	BP 470/40	BP 525/50
Zeiss Filter set 43 (dsRed)	BP550/25	BP 605/70
Zeiss Filter set 49 (DAPI)	G365nm	BP 445/50
Zeiss filter set 50 (Cy5)	BP 640/30	BP 690/50

Detectors

• Zeiss AxioCam 503 Mono
• Zeiss AxioCam ICc 1 (color camera)

DESCRIPTION
Zeiss Stereo Lumar V12 is a fully automated stereoscope with fluorescence capabilities. It allows imaging of whole small animals (e.g. Zebrafish and c. elegans), as well as cell cultures in petri dishes. Magnifications range is equivalent to ~1-12x, with variable zoom.

Microscope Body

• Zeiss Lumar.V12 stereoscope body

Objectives

• Neo Lumar S 0.8x

Light Source

• Transmitted and reflected light source
• HBO100 fluorescence arc lamp

Filter Sets

• Zeiss Filter set 38 (GFP)
• Zeiss Filter set 20 (Rhodamine)
• Zeiss Filter set 01 (UV)

Detectors

• Zeiss AxioCam MRm

DESCRIPTION
The microinjector arm module is installed on the Nikon TE2000U microscope. This system is suitable for micromanipulation and microinjection applications.

Microscope Body

• Nikon TE2000U

Objectives

• Plan Fluor 10x/0.3
• Plan Fluor 20x/0.45
• LWD 20x/0.4
• Plan Fluor 40x/0.6
• LWD 40x/0.55
• Plan Fluor 60x/0.7

Light Source

• Mercury lamp

Excitation wavelengths

• 457nm, 488nm, 514nm
• 561nm
• 633nm

DESCRIPTION

The LSM 980 Airyscan 2 is an ideal platform for fast, gentle, super-resolution imaging. It is built on a fully motorized Axio Observer 7 inverted microscope stand with Z-peizo, environmental chamber with temperature control and uses the ZEN Blue 3.4 software for acquisition and analysis. It is equipped with a 32 channel GaAsP detector, 2 PMTs and an Airyscan 2 detector. The multiplex mode can perform parallel pixel acquisition, which can accelerate acquisition speed, up to 8x faster than normal confocal imaging.

 SPECIFICATIONS

Microscope body

• Axio Observer 7
• Environmental Chamber for temperature control

 Objectives

1. EC Plan-NeoFluar 2.5x/0.085
2. Plan-Apochromat 10x/0.45
3. Plan-Apochromat 25x/0.8
4. Plan-Apochromat 40x/1.30 Oil DIC
5. Plan-Apochromat 63x/1.40 Oil

 Light Source

• Halogen lamp for transmitted light
• X-Cite 120 illuminator for reflected light
• Lasers:

- Diode Laser 405nm,445nm,488nm,514nm,639nm

- DPSS laser 561nm, 594nm

 Detectors

• 32 channel GsAsp detector
• 2 standard PMTs
• Airyscan 2 detector

 Software

• Zen 3.4

DESCRIPTION
The IMARIS single full software is installed on this workstation. This program allows the visualisation, analysis and interpretation of microscopy data. With IMARIS, you can perform automated spots and surface detection, cell segmentation, motion tracking, co-localisation and cell lineage studies etc.