Integrative Microbiology Laboratory

The research focus on particular deep-sea microbiology and the latest “omic” technologies that are essential for deriving a clear understanding of microbial communities and ecosystem function.

 

Equipment: Environmental Anaerobic Chamber

Model/Brand: 300-2-SP Bactron

Specifications

  • 60% faster airlock cycle with 3,4 or 5 auto cycle iterations
  • Auto-sleeve cycle with integrated HEPA filter, 40% faster sleeve cycle

Applications

Samples can be prepared, cultured, and inspected in the oxygen-free chamber

 

Equipment: PCR Cabinet

Model/Brand: PCR1500 BIOBASE

Specifications

  • HEPA Filter: 99.999% efficiency at 0.3μm
  • Airflow Velocity: 0.3-0.5m/s, speed adjustable

Applications

Flexibility to support numerous applications

 

Equipment: PCR Cabinet

Model/Brand: PCR800 BIOBASE

Specifications

  • HEPA Filter: 99.999% efficiency at 0.3μm
  • Airflow Velocity: 0.3-0.5m/s, speed adjustable

Applications

Flexibility to support numerous applications, mainly for PCR preparations

 

Equipment: DNA/RNA UV-Cleaner Box

Model/Brand: UVC/T-AR BioSan

Specifications

  • UV radiation level: 18 mW / cm2 / sec
  • Radiation type: UV (λ = 253.7 nm), ozone-free
  • Digital time setting of direct UV exposure: 1 min–24 hrs / non–stop (increment 1 min)
  • UV-recirculator: 1x25W (efficiency >99% per 1 hour)
  • Daylight lamp (for working area illumination): 1 x TLD-15W

Applications

For DNA and RNA preparation and decontamination

 

Equipment: Hybridization Oven with built-in Shaker

Model/Brand: ProBlot 12S Labnet

Specifications

  • Bottle capacity (large/small): 12/24
  • Rotisserie/rocker speed: 4 to 20 rpm
  • Shaker speed: 10 to 300 rpm
  • Temperature Range: Ambient +5° C to 80° C
  • Temperature uniformity: ±0.5°C

Applications

Medium to high throughput laboratories with its 12 bottle capacity

 

Equipment: Refrigerated Benchtop Centrifuge

Model/Brand: ST8R Thermo Scientific

Specifications

  • Maximum Speed: 17,850 RPM (fixed angle rotor)
  • Minimum Speed: 300 RPM
  • Maximum RCF: 30,279 x g
  • Maximum Kinetic Energy: 10.1 kJ
  • Running Time: 99 h 59 min 50 sec, hold
  • Temperature Range; -10°C to 40°C

Applications

Flexibility to support numerous applications.

 

Equipment: Thermo Shaker

Model/Brand: TS-100  BioSan

Specifications

  • Temperature setting range: +25°C to +100°C
  • Temperature control range: 5°C above ambient to +100°C
  • Temperature setting resolution: 0.1°C
  • Temperature stability: ±0.1°C
  • Temperature uniformity over the block at +37°C: ±0.1°C
  • Speed control range: 250–1400 rpm
  • Digital time setting: 1 min–96 hrs (1 min increment)

Applications

For DNA analysis sample preparation, for extraction of proteins, polysaccharides, lipids and other cellular components.

 

Equipment: Digital Orbital Shaker

Model/Brand: SK-O180-S DLAB

Specifications

  • Max. load capacity (with platform): 3kg
  • Speed control range: 250–1400 rpm
  • Digital time setting: 1 min–96 hrs (1 min increment)

Applications

Flexibility to support numerous applications.

 


Equipment: Thermal Cycler

Model/Brand: SimpliAmp

Specifications

  • For Use With (Equipment): GeneAmp 2700, GeneAmp 9700, Veriti Thermal Cycler, 2720 Thermal Cycler
  • Block Configurations: 96-Well, 0.2 mL VeriFlexs
  • Max. Ramp Rate: 4°C/Sec (Block), 3°C/Sec (Sample)
  • Thermal Uniformity: <0.5°C (30 Sec After Reaching 95°C)
  • Thermal Accuracy: ±0.0.25°C (35°C to 99.9°C)
  • Thermal Range: 0 to 100°C

Applications

For PCR

 

Equipment: Real-time PCR system

Model/Brand: STEPONEPLUS Life Technologies

Specifications

  • Detection Method: SYBR, Primer-Probe Detection
  • Format: 96-well plate, 8-tube strips, 0.1 ml tubes
  • Peak Block Ramp Rate: 4.6°C⁄sec
  • Reaction Volume Range: 10-30 µl (Standard curve experiments: 40 µl in standard mode is validated)
  • Sample Ramp Rate: Fast mode: ± 2.2°C⁄sec, Standard mode: ± 1.6°C⁄sec
  • Temperature Range: 4-100°C
  • Temperature Uniformity: 0.25°C (35 to 95°C) of setpoint/display temperature

Applications

Quantitative Real-Time PCR results for a variety of genomic research applications. Real-Time PCR applications include SNP Genotyping, Gene Expression Analysis, MicroRNA Expression, Protein Expression, Translocation Analysis, Gene Detection and Viral Load Analysis

 

Equipment: Gradient Thermal Cycler

Model/Brand: PTC-200 MJ Research

Specifications

  • Thermal Range: 5° to 105°C, but no more than 30°C below ambient temperature 
  • Thermal Accuracy: ±0.3°C of programmed target at 90°C- NIST-traceable
  • Thermal homogeneity: ±0.4°C well-to-well within 30 seconds of arrival at 90°
  • Thermal Uniformity: 90°C 

Applications

Gradient DNA Engine Thermal Cycler

 

Equipment: Flow Cytometer

Model/Brand: CYTOFLEX BECKMAN COULTER

Specifications

  • Detection: Photodiodes
  • Violet Side Scatter Resolution: (VSSC) <200 nm
  • Blue Laser Wavelength: 488 nm; Power: 50 mW; Beam spot size: 5 x 80 μm
  • Red Laser Wavelength: 638 nm; Power: 50 mW; Beam spot size: 5 x 80 μm
  • Violet Laser Wavelength: 405 nm; Power: 80 mW; Beam spot size: 5 x 80 μm
  • Flow Cell dimensions: 420 μm x 180 μm internal diameter
  • Signal Processing: Fully digital system with 7 decade data display
  • Carryover: Single Tube Format < 1.0%; Plate Loader Format < 0.5%

Applications

For data acquisition and analysis

 

Equipment: Planetary Ball Mill Machine

Model/Brand: TMAX-XQM-0.4

Specifications

  • Grinding tank volume (L): 0.1L*4
  • Feeding material size: Soil material ≤10mm, other materials ≤3mm
  • Discharge material size: Min 0.1um
  • Rotating speed: Revolution 335r / min, Rotation 670r / min
  • Speed ratio: (revolution: rotation) 1:2

Applications

Different materials can be grinding and mixing by the mill with dry or wet method. The size of ground powder can be reduced to as small as 0.1 micrometer.

 

Equipment: E-Gel Imager

Model/Brand: Life Technologies

Specifications

  • Excitation light source: UV light–312 nm; Blue light–470 nm
  • Camera  
  • Type: CCD      
  • Resolution: 1,296 (H) x 964(V); 1.3 megapixels      
  • Dynamic range: 56.77 DB      
  • Exposure time: 0.01 milliseconds to 10 seconds
  • Optics 
  • Super bright lens: F/1.4 improved lens; Optical zoom 6-12 mm      
  • Field of view: 11 x 14 cm      
  • Emission filter: Orange filter (ethidium bromide and SYBR Safe DNA Gel Stain), Green filter (Fluorescein, SYBR Gold, SYBR Green), Red filter (Qdot 625)

Applications

For the documentation and densitometric analysis of stained nucleic acids and proteins in agarose and polyacrylamide gels, including E-Gel agarose gels and Invitrogen Novex NuPAGE acrylamide gels.