Animal work that requires IACUC approval

Research or teaching protocols involving the use of animals require NTU-IACUC approval before work on these animals can commence. 

These include work done on all live vertebrates such as:

  • mammals
  • fish
  • amphibians
  • reptiles
  • and invertebrates that are cephalopods (e.g. squid, octopuses, etc).

For work on embryos and foetuses of the above animals, IACUC approval is required when they have reached the developmental stage where they begin to feel pain. At NTU, IACUC approval is required for use of:

Fertilised chicken eggs: From 7 incubation days
Zebrafish embryos: From 5 days post fertilization (d.p.f)
Mice & Rats embryos: From 15 gestation days
Marmoset foetuses: From 100 gestation days

In addition, animals involved in the production of embryos/foetuses must be covered by an IACUC approved protocol. For work involving embryos of all other animals covered by the NACLAR Guidelines, researchers can write to IACUC@ntu.edu.sg to check if IACUC approval for the project is required.

The euthanasia of animals mentioned above specifically for the purpose of obtaining tissues, requires IACUC review and approval. Also, all postmortem work done as part of a study’s objectives, (e.g. tissue harvesting, details of tissues harvested, what downstream analysis will be done, etc.) must be described in a protocol application.

 For further queries, please write to IACUC@ntu.edu.sg.